RESUMO
The current study deals with the setting up of a new tool that enables the benign versus the malignant nature of colorectal epithelium to be determined early and accurately. The objective is to determine a different biologic characteristic between normal and malignant colorectal tissue, which is the site and the level of expression of the T glycoepitope (Thomsen-Friedenreich antigen). It was characterized in a series of 62 colorectal samples, including 31 normal (without pathologic lesion) and 31 cancerous (mostly moderately or poorly differentiated) tissue sections. The glycoconjugate expression was demonstrated by lectin-histochemistry, using PNA lectin. The binding patterns of the lectin were determined in both columnar and goblet cells, from normal and malignant colorectal tissue. The results show that specific and different glycochemical staining patterns could be identified between benign and malignant epithelium. The data of the cytostructural localization were submitted to statistical analyses, which strongly suggested the association between the patterns of expression of the T antigen and the degree of the tissue differentiation. The methodology developed can be applied directly in routine diagnosis and it has an important prognostic value.
Assuntos
Antígenos de Neoplasias/análise , Antígenos Glicosídicos Associados a Tumores/análise , Carcinoma/imunologia , Neoplasias Colorretais/imunologia , Células Epiteliais/imunologia , Carcinoma/química , Neoplasias Colorretais/química , Células Epiteliais/química , Feminino , Humanos , Imuno-Histoquímica , Lectinas , Masculino , Pessoa de Meia-IdadeRESUMO
The current study deals with the setting up of a new tool that enables the benign versus the malignant nature of colorectal epithelium to be determined early and accurately. The objective is to determine a different biologic characteristic between normal and malignant colorectal tissue, which is the site and the level of expression of the T glycoepitope (Thomsen-Friedenreich antigen). It was characterized in a series of 62 colorectal samples, including 31 normal (without pathologic lesion) and 31 cancerous (mostly moderately or poorly differentiated) tissue sections. The glycoconjugate expression was demonstrated by lectin-histochemistry, using PNA lectin. The binding patterns of the lectin were determined in both columnar and goblet cells, from normal and malignant colorectal tissue. The results show that specific and different glycochemical staining patterns could be identified between benign and malignant epithelium. The data of the cytostructural localization were submitted to statistical analyses, which strongly suggested the association between the patterns of expression of the T antigen and the degree of the tissue differentiation. The methodology developed can be applied directly in routine diagnosis and it has an important prognostic value.
RESUMO
El tejido epitelial gastrointestinal normal presenta estructuras glicoesfingolipídicas que son propias de los antígenos ABH, las cuales confieren propiedades biológicas esenciales, dirigen el recambio y el tráfico transcelular y tienen gran importancia para la interacción entre células durante el desarrollo, crecimiento y diferenciación. Está descripto que la glicosilación aberrante es un atributo común del crecimiento neoplásico y uno de los principales determinantes del fenómeno relacionado con el cáncer, como es el crecimiento invasivo de la metástasis. El objetivo de este trabajo fue estudiar la expresión de los antígenos ABH en células epiteliales de neoplasias gastrointestinales. Se trabajó con catorce muestras de tumores gastrointestinales en tacos de parafina aplicando la técnica SRCA (Specific Red Cell Adherence). Se demostró que en 13 de las 14 muestras hubo pérdida total o parcial, o cambio de la expresión antigénica
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Neoplasias Gastrointestinais/diagnóstico , Imuno-Histoquímica/métodos , Isoantígenos , Biomarcadores Tumorais/isolamento & purificação , Neoplasias do Colo/diagnóstico , Neoplasias Colorretais/diagnóstico , Neoplasias Colorretais/imunologia , Glucosiltransferases , Invasividade Neoplásica/imunologia , Marcadores Genéticos/imunologia , Biomarcadores Tumorais , Sistema ABO de Grupos Sanguíneos/imunologia , Neoplasias Gástricas/diagnóstico , Neoplasias Gástricas/imunologia , Transformação Celular Neoplásica/genética , Transformação Celular Neoplásica/imunologiaRESUMO
El tejido epitelial gastrointestinal normal presenta estructuras glicoesfingolipídicas que son propias de los antígenos ABH, las cuales confieren propiedades biológicas esenciales, dirigen el recambio y el tráfico transcelular y tienen gran importancia para la interacción entre células durante el desarrollo, crecimiento y diferenciación. Está descripto que la glicosilación aberrante es un atributo común del crecimiento neoplásico y uno de los principales determinantes del fenómeno relacionado con el cáncer, como es el crecimiento invasivo de la metástasis. El objetivo de este trabajo fue estudiar la expresión de los antígenos ABH en células epiteliales de neoplasias gastrointestinales. Se trabajó con catorce muestras de tumores gastrointestinales en tacos de parafina aplicando la técnica SRCA (Specific Red Cell Adherence). Se demostró que en 13 de las 14 muestras hubo pérdida total o parcial, o cambio de la expresión antigénica (AU)
